| St Andrews Bus Station | 13:25:00 | |
| St Andrews John Burnet Hall | 13:25:01 | |
| St Andrews Old Course Hotel | 13:25:02 | |
| St Andrews Strathtyrum Golf Course | 13:27:00 | |
| Easter Kincaple Farm | 13:29:00 | |
| Kincaple Edenside | 13:31:00 | |
| Guardbridge Hotel | 13:32:00 | |
| Guardbridge Cupar Road | 13:33:00 | |
| Guardbridge Seggie Farm | 13:33:01 | |
| Clayton Bruckley Farm | 13:35:00 | |
| Dairsie Caravan Park | 13:36:00 | |
| Dairsie Pitcairn Park | 13:39:00 | |
| Dairsie Primary School | 13:39:01 | |
| Cupar Foxton Farm Road End | 13:42:00 | |
| Cupar Trading Estate | 13:44:00 | |
| Cupar Thomastoun Drive | 13:45:00 | |
| Cupar Bowling Green Road | 13:45:01 | |
| Cupar Railway Station | 13:47:00 | |
| Cupar Tesco Supermarket | 13:47:01 | |
| Cupar Cow Brae | 13:47:02 | |
| Cupar Knox Gardens | 13:48:00 | |
| Cupar Tarvit Mill | 13:50:00 | |
| Springfield Edenwood Farm | 13:51:00 | |
| Springfield Clushford Toll | 13:51:01 | |
| Pitlessie Cults Mill Road End | 13:53:00 | |
| Pitlessie Cults Road End | 13:53:01 | |
| Pitlessie The Wynd | 13:55:00 | |
| Balmalcolm Main Road | 13:57:00 | |
| Kettlebridge Kettlehill Road End | 13:57:01 | |
| Kettlebridge Mid Street | 13:58:00 | |
| Muirhead Welltree Junction | 14:00:00 | |
| Muirhead Village | 14:02:00 | |
| Glenrothes Kirkforthar Feus | 14:03:00 | |
| Glenrothes Gateside Cottages | 14:04:00 | |
| Queensway Auchmuty High School | 14:08:00 | |