| Gloucester Transport Hub | 16:00:00 | |
| Kingsholm St Mark's Church | 16:02:00 | |
| Kingsholm Rugby Ground | 16:03:00 | |
| Kingsholm Denmark Road | 16:03:01 | |
| Longford Gambier Parry Gardens | 16:05:00 | |
| Kingsholm Fairmile Gardens | 16:05:01 | |
| Longford Fircroft Road | 16:07:00 | |
| Longford Sherwood Green | 16:08:00 | |
| Longford Crescentdale | 16:09:00 | |
| Longford Park | 16:10:00 | |
| Longlevens Brionne Way | 16:11:00 | |
| Longlevens Oxstalls Way | 16:12:00 | |
| Longlevens Crossroads | 16:13:00 | |
| Longlevens Brooklands Park | 16:14:00 | |
| Longlevens Allotments | 16:15:00 | |
| Innsworth Technology Park | 16:16:00 | |
| Innsworth House Farm | 16:17:00 | |
| Innsworth Frog Furlong Lane | 16:18:00 | |
| Innsworth Imjin Barracks | 16:19:00 | |
| Churchdown Shepherds Way | 16:20:00 | |
| Churchdown Wisteria Way | 16:21:00 | |
| Churchdown St John's Church | 16:22:00 | |
| Churchdown St John's Avenue Shops | 16:22:01 | |
| Churchdown Morley Avenue | 16:23:00 | |
| Churchdown Winston Road | 16:25:00 | |
| Churchdown Cordingley Close | 16:26:00 | |
| Churchdown Station Road | 16:26:01 | |
| Churchdown The Old Elm Inn | 16:27:00 | |
| Churchdown Albemarle Road | 16:28:00 | |
| Churchdown Chosen Hill School | 16:29:00 | |
| Churchdown Rugby Club | 16:30:00 | |
| Badgeworth Brookfield Road | 16:32:00 | |
| The Reddings Reddings Farm | 16:33:00 | |
| The Reddings Chestnut Nurseries | 16:34:00 | |
| The Reddings Brock Close | 16:35:00 | |
| The Reddings Leyson Road | 16:36:00 | |
| The Reddings Reddings Park | 16:37:00 | |
| Hatherley Robert Burns Avenue | 16:38:00 | |
| Hatherley Post Office | 16:40:00 | |
| Hatherley Harrington Drive | 16:41:00 | |
| Hatherley Shelburne Road | 16:43:00 | |
| Hatherley Warden Hill Road | 16:43:01 | |
| Hatherley Court Gardens | 16:45:00 | |
| Cheltenham Lansdown Walk | 16:46:00 | |
| Cheltenham Rotunda | 16:47:00 | |
| Cheltenham Imperial Square | 16:49:00 | |
| Cheltenham Promenade | 16:50:00 | |