| Glenrothes Bus Station | 14:06:00 | |
| Glenrothes Auchmuty Road | 14:06:01 | |
| Queensway Morrisons Supermarket | 14:07:00 | |
| Queensway Auchmuty High School | 14:07:01 | |
| Glenrothes Gateside Cottages | 14:11:00 | |
| Freuchie Channelhall | 14:14:00 | |
| Freuchie Road End | 14:15:00 | |
| Freuchie Riggs Farm | 14:16:00 | |
| Ladybank Church Street | 14:20:00 | |
| Ladybank Railway Station | 14:20:01 | |
| Ladybank Hill Street | 14:20:02 | |
| Ladybank Beech Avenue | 14:21:00 | |
| Ladybank Pine Lodge | 14:21:01 | |
| Ladybank Annsmuir Caravan Park | 14:22:00 | |
| Ladybank Edensmuir Forest | 14:23:00 | |
| Ladybank Roundabout | 14:24:00 | |
| Bow of Fife Post Office | 14:25:00 | |
| Bow of Fife Scottish Deer Centre | 14:26:00 | |
| Springfield Hammerhead toll | 14:27:00 | |
| Stratheden Uthrogle Mills | 14:27:01 | |
| Stratheden Hangman's Lane | 14:28:00 | |
| Cupar The Drum | 14:30:00 | |
| Cupar Elmwood College | 14:30:01 | |
| Cupar West Park Road | 14:32:00 | |
| Cupar West Port | 14:33:00 | |
| Cupar St Columbas Primary School | 14:34:00 | |
| Cupar Provost Wynd | 14:36:00 | |
| Cupar Crossgate | 14:38:00 | |
| Cupar Railway Station | 14:40:00 | |